Anti-Keap1 antibody
| Name | Anti-Keap1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab166958 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Rat, Human, Sheep, Rabbit, Bovine, Pig, Chimpanzee, Ape, Hamster, Orangutan |
| Antigen | Synthetic peptide corresponding to Human Keap1 aa 550 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | KEAP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab166958 stained MCF7 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab166958 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-Keap1 antibody (ab166958) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissueLane 2 : Skeletal Muscle (Rat) Tissue LysateLane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue with Immunising peptide at 1 µg/mlLane 4 : Skeletal Muscle (Rat) Tissue Lysate with Immunising peptide at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.