Anti-KHSRP antibody
| Name | Anti-KHSRP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83291 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human, Zebrafish, Mouse, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog |
| Antigen | Synthetic peptide within residues: WEEYYKKQAQ VATGGGPGAP PGSQPDYSAA WAEYYRQQAA YYGQTPGPGG , corresponding to internal sequence amino acids 648-697 of Human KHSRP (NP_003676) Run BLAST with Run BLAST with |
| Blocking Peptide | Human KHSRP peptide |
| Description | Rabbit Polyclonal |
| Gene | KHSRP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling KHSRP with ab83291 at 4.0-8.0µg/ml. Positive staining shown in cardiac cells. Magnification: 400X.
Anti-KHSRP antibody (ab83291) at 1 µg/ml + 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
ICC/IF image of ab83291 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83291, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-KHSRP antibody (ab83291) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate at 20 µgLane 3 : Zebrafish heart homogenate at 10 µgLane 4 : Zebrafish liver homogenate at 10 µgLane 5 : Zebrafish skeletal muscle homogenate at 10 µgSecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway. - ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway.
Adachi S, Homoto M, Tanaka R, Hioki Y, Murakami H, Suga H, Matsumoto M, Nakayama KI, Hatta T, Iemura S, Natsume T. Nucleic Acids Res. 2014 Sep;42(15):10037-49.
Electrophilic tuning of the chemoprotective natural product sulforaphane. - Electrophilic tuning of the chemoprotective natural product sulforaphane.
Ahn YH, Hwang Y, Liu H, Wang XJ, Zhang Y, Stephenson KK, Boronina TN, Cole RN, Dinkova-Kostova AT, Talalay P, Cole PA. Proc Natl Acad Sci U S A. 2010 May 25;107(21):9590-5. doi: