Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121643 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
All lanes : Anti-KIAA1045 antibody (ab121643) at 1/250 dilutionLane 1 : RT4 cell lysateLane 2 : U 251 MG cell lysateLane 3 : Human Plasma lysateLane 4 : Human Liver lysateLane 5 : Human Tonsil lysate10-20% SDS PAGE
ab121643 at 1/500 dilution staining KIAA1045 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.