Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122425 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
All lanes : Anti-KIAA1598 homolog antibody (ab122425) at 1/250 dilutionLane 1 : RT4 cell lysateLane 2 : U-251 MG cell lysateLane 3 : Human Plasma lysateLane 4 : Human Liver lysateLane 5 : Human Tonsil lysatedeveloped using the ECL technique10-20% SDS PAGE
ab122425 at 1/3000 dilution staining KIAA1598 homolog in Paraffin-embedded Human prostate tissue by Immunohistochemistry.