Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/20000 dilution + 293 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling KIF1A with ab180153 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.
Western blot analysis of 293 cell lysate immunoprecipitated using ab180153 at 1/30 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling KIF1A with ab180153 at 1/150 dilution (red), compared to a Rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.
Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution + Human fetal brain lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution