Anti-Kinesin 5B antibody

• Supplier: Abcam
• Catalog: ab5629
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to Human Kinesin 5B aa 376-396
• Description: Rabbit Polyclonal
• Gene: KIF5B
• Conjugate: Unconjugated

Product images

ab5629 labelling Kinesin 5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.

ab5629 at 1/200 dilution staining the mouse oligodendrocyte cell line, Olineu, by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated secondary antibody. This image is courtesy of an Abreview submitted by Katarina Trajko on 22 February 2006.See Abreview

Product References

Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ - Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+

Barry J, Xu M, Gu Y, Dangel AW, Jukkola P, Shrestha C, Gu C. J Cell Sci. 2013 May 1;126(Pt 9):2027-41.

Microtubule motors mediate endosomal sorting by maintaining functional domain - Microtubule motors mediate endosomal sorting by maintaining functional domain

Hunt SD, Townley AK, Danson CM, Cullen PJ, Stephens DJ. J Cell Sci. 2013 Jun 1;126(Pt 11):2493-501.

Dynein and kinesin regulate stress-granule and P-body dynamics. - Dynein and kinesin regulate stress-granule and P-body dynamics.

Loschi M, Leishman CC, Berardone N, Boccaccio GL. J Cell Sci. 2009 Nov 1;122(Pt 21):3973-82.

Depletion of kinesin 5B affects lysosomal distribution and stability and induces - Depletion of kinesin 5B affects lysosomal distribution and stability and induces

Cardoso CM, Groth-Pedersen L, Hoyer-Hansen M, Kirkegaard T, Corcelle E, Andersen JS, Jaattela M, Nylandsted J. PLoS One. 2009;4(2):e4424.

GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: - GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins:

Brickley K, Smith MJ, Beck M, Stephenson FA. J Biol Chem. 2005 Apr 15;280(15):14723-32. Epub 2005 Jan 11.