Anti-Kinesin 5C antibody

• Supplier: Abcam
• Catalog: ab5630
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ICC/IF ICC/IF WB IP
• Species Reactivities: Rat, Human
• Antigen: Synthetic peptide corresponding to Human Kinesin 5C aa 938-957
• Description: Rabbit Polyclonal
• Gene: KIF5C
• Conjugate: Unconjugated

Product images

ab5630 staining Kinesin 5C in COS-7 cells by Immunocytochemistry/ Immunofluorescence.GFP-Kif5c behaviour in living cells. Cropped still images from a time-lapse recording of GFP-Kif5c puncta (green) moving along a MT labelled with mCherry–-tubulin (red) in a live COS-7 cell. The GFP-Kif5c puncta move from right to left. Scale bar, 2 µm. The graph shows the respective velocity of the GFP-Kif5c puncta seen over time, demonstrating the rapid changes in velocity of those puncta.

IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Mutations in TUBG1, DYNC1H1, KIF5C and KIF2A cause malformations of cortical - Mutations in TUBG1, DYNC1H1, KIF5C and KIF2A cause malformations of cortical

Poirier K, Lebrun N, Broix L, Tian G, Saillour Y, Boscheron C, Parrini E, Valence S, Pierre BS, Oger M, Lacombe D, Genevieve D, Fontana E, Darra F, Cances C, Barth M, Bonneau D, Bernadina BD, N'guyen S, Gitiaux C, Parent P, des Portes V, Pedespan JM, Legrez V, Castelnau-Ptakine L, Nitschke P, Hieu T, Masson C, Zelenika D, Andrieux A, Francis F, Guerrini R, Cowan NJ, Bahi-Buisson N, Chelly J. Nat Genet. 2013 Jun;45(6):639-47.

Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules - Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules

Dunn S, Morrison EE, Liverpool TB, Molina-Paris C, Cross RA, Alonso MC, Peckham M. J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95.

Mapping the GRIF-1 binding domain of the kinesin, KIF5C, substantiates a role for - Mapping the GRIF-1 binding domain of the kinesin, KIF5C, substantiates a role for

Smith MJ, Pozo K, Brickley K, Stephenson FA. J Biol Chem. 2006 Sep 15;281(37):27216-28. Epub 2006 Jul 11.

GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: - GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins:

Brickley K, Smith MJ, Beck M, Stephenson FA. J Biol Chem. 2005 Apr 15;280(15):14723-32. Epub 2005 Jan 11.