All lanes : Anti-Kinesin 5C antibody [EPR16224-32] (ab193352) at 1/10000 dilutionLane 1 : Neuro-2a lysateLane 2 : Human fetal brain lysateLane 3 : SH-SY5Y lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Kinesin 5C antibody [EPR16224-32] (ab193352) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLane 3 : C6 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human testis tissue sections labeling Kinesin 5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue sections labeling Kinesin 5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling Kinesin 5C using ab193352 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.