All lanes : Anti-Kinesin Heavy Chain antibody (ab88671) at 1/1000 dilutionLane 1 : HEK293 cell lysateLane 2 : 293T cell lysate
ICC/IF image of ab88671 stained CACO-2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab88671 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab88671 at 1/100 dilution staining Kinesin Heavy Chain in human fetal kidney by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.