Anti-Kir2.1 antibody
| Name | Anti-Kir2.1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab65796 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide derived from the N terminal domain of human Kir2 |
| Description | Rabbit Polyclonal |
| Gene | KCNJ2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab65796 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65796, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab65796 staining Kir2.1 in human colon.Left panel: with primary antibody at 1:1000 dilution. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Chronic depression of hypothalamic paraventricular neuronal activity produces - Chronic depression of hypothalamic paraventricular neuronal activity produces
Geraldes V, Goncalves-Rosa N, Liu B, Paton JF, Rocha I. Exp Physiol. 2014 Jan;99(1):89-100.
Characterisation of K+ currents in the C8-B4 microglial cell line and their - Characterisation of K+ currents in the C8-B4 microglial cell line and their
Moussaud S, Lamodiere E, Savage C, Draheim HJ. Cell Physiol Biochem. 2009;24(3-4):141-52.