All lanes : Anti-KIR2DL4 antibody (ab100996) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.