All lanes : Anti-Kir6.2 antibody (ab79171) at 1/500 dilutionLane 1 : extracts from HeLa cells treated with heat shockLane 2 : extracts from NIH3T3 cells treated with heat shockLane 3 : extracts from NIH3T3 cells treated with heat shock with immunizing peptide at 10 µgLysates/proteins at 20 µg per lane.
Immunohistochemical analysis of Kir6.2 in paraffin embedded human brain tissue using a 1/50 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.
Immunofluorescence analysis of Kir6.2 in HeLa cells, using a 1/500 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.