All lanes : Anti-KLC3 antibody (ab101188) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lane 3 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)Lane 4 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab101188 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101188, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.