All lanes : Anti-KLF13 antibody (ab88536) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear LysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate (ab14860)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.