Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab182163 at 1/50 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling KLHL6 with ab182163 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI.
Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + A549 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + Human fetal kidney lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution