All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/2000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysateLane 3 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/1000 dilutionLane 1 : C6 (Rat glial tumor cells) whole cell lysateLane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 3 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-KLLN antibody [EPR16934] (ab197892) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution