Anti-KMT1C / G9a antibody
| Name | Anti-KMT1C / G9a antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31874 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide derived from within residues 1 - 100 of Mouse KMT1C/ G9a |
| Description | Rabbit Polyclonal |
| Gene | EHMT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-KMT1C / G9a antibody (ab31874) at 1 µg/ml (blocked with 3% milk)Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell LysateLane 2 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutionPerformed under reducing conditions.
IHC image of ab31874 staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31874, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Rb and p130 control cell cycle gene silencing to maintain the postmitotic - Rb and p130 control cell cycle gene silencing to maintain the postmitotic
Sdek P, Zhao P, Wang Y, Huang CJ, Ko CY, Butler PC, Weiss JN, Maclellan WR. J Cell Biol. 2011 Aug 8;194(3):407-23.
Gestational choline supply regulates methylation of histone H3, expression of - Gestational choline supply regulates methylation of histone H3, expression of
Davison JM, Mellott TJ, Kovacheva VP, Blusztajn JK. J Biol Chem. 2009 Jan 23;284(4):1982-9.