Western blot analysis on immunoprecipitation. IP sample: 2 mg of HeLa whole cell lysate/extract. Lanes: 1) 40 μg of HeLa whole cell lysate/extract. 2) control with 2 μg of pre-immune rabbit IgG. 3) immunoprecipitation of KMT1C / G9a with 2 μg of ab183889. KMT1C / G9a immunoprecipitate was detected by ab183889 at a 1/1000 dilution.
Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde for 5 minutes, labeling KMT1C / G9a using ab183889 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded HeLa xenograft tissue, labeling KMT1C / G9a using ab183889 at a 1/500 dilution.
All lanes : Anti-KMT1C / G9a antibody (ab183889) at 1/1000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : A431 whole cell lysate/extractLane 3 : HeLa whole cell lysate/extractLane 4 : HepG2 whole cell lysate/extractLane 5 : A375 whole cell lysate/extractLysates/proteins at 30 µg per lane.