Anti-KMT1C / G9a antibody - ChIP Grade
| Name | Anti-KMT1C / G9a antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab40542 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ChIP IHC-P WB |
| Species Reactivities | Human |
| Antigen | Recombinant fusion protein, corresponding to amino acids 831-1001 of Human G9a |
| Description | Rabbit Polyclonal |
| Gene | EHMT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-KMT1C / G9a antibody - ChIP Grade (ab40542) at 1/2500 dilutionLane 1 : Untransfected HeLa cell extractLane 2 : G9a transfected HeLa cell extract
ab40542 (1:2000) staining KMT1C in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark - Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark
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