ab84989 at 1/250 dilution staining KMT6 / EZH2 in serial sections of human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right).
ab84989 at 1/250 dilution staining KMT6 / EZH2 in serial sections of mouse teratoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right).
ab84989 at 1/250 dilution staining KMT6 / EZH2 in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
ICC/IF image of ab84989 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84989, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.