Anti-KSR2 antibody
| Name | Anti-KSR2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab72753 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF IP |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide from an internal region of human KSR2 |
| Description | Rabbit Polyclonal |
| Gene | KSR2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-KSR2 antibody (ab72753) at 1/500 dilutionLane 1 : 293 cell extractLane 2 : 293 cell extract with immunising peptide at 10 µgLysates/proteins at 10 µg per lane.
Immunofluorescence analysis of KSR2 in HeLa cells, using ab72753 at 1/500 dilution. Right panel was also treated with immunising peptide.
![KSR2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to KSR2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72753.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 108kDa; KSR2](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14197_KSR2-Primary-antibodies-ab72753-1.jpg)
KSR2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to KSR2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72753.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 108kDa; KSR2