Anti-Ku80 antibody [111]
| Name | Anti-Ku80 antibody [111] |
|---|---|
| Supplier | Abcam |
| Catalog | ab80830 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 111 |
| Applications | WB IP FC ICC/IF ICC/IF |
| Species Reactivities | Human, Monkey |
| Antigen | Nuclei from plasmacytoid 2p68 cells ((Human B lymphocytes) |
| Description | Mouse Monoclonal |
| Gene | XRCC5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab80830 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80830, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Murine monoclonal antibodies specific for conserved and non-conserved antigenic - Murine monoclonal antibodies specific for conserved and non-conserved antigenic
Wang J, Chou CH, Blankson J, Satoh M, Knuth MW, Eisenberg RA, Pisetsky DS, Reeves WH. Mol Biol Rep. 1993 Jun;18(1):15-28.
Use of monoclonal antibodies for the characterization of novel DNA-binding - Use of monoclonal antibodies for the characterization of novel DNA-binding
Reeves WH. J Exp Med. 1985 Jan 1;161(1):18-39.