Anti-Kv1.1 potassium channel antibody

• Supplier: Abcam
• Catalog: ab32433
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB IHC-F
• Species Reactivities: Mouse, Rat, Chicken, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Rat Kv1
• Description: Rabbit Polyclonal
• Gene: KCNA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Kv1.1 potassium channel antibody (ab32433) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Heart (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab32433 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32433, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

IHC FoFR image of ab32433 stained sections of rat dorsal root ganglion (20 um). The tissues was from perfusion fixed animals with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.See Abreview

Product References

Clinical, genetic, neurophysiological and functional study of new mutations in - Clinical, genetic, neurophysiological and functional study of new mutations in

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Adult medulloblastoma comprises three major molecular variants. - Adult medulloblastoma comprises three major molecular variants.

Remke M, Hielscher T, Northcott PA, Witt H, Ryzhova M, Wittmann A, Benner A, von Deimling A, Scheurlen W, Perry A, Croul S, Kulozik AE, Lichter P, Taylor MD, Pfister SM, Korshunov A. J Clin Oncol. 2011 Jul 1;29(19):2717-23.

Switch of voltage-gated K+ channel expression in the plasma membrane of - Switch of voltage-gated K+ channel expression in the plasma membrane of

Varga Z, Juhasz T, Matta C, Fodor J, Katona E, Bartok A, Olah T, Sebe A, Csernoch L, Panyi G, Zakany R. PLoS One. 2011;6(11):e27957.