Anti-L1CAM antibody [2C2]
| Name | Anti-L1CAM antibody [2C2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24345 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2C2 |
| Applications | ICC/IF ICC/IF IHC-F FC WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | chicken NgCAM protein (ab24345 detects the C-terminus portion of the protein that is conserved with mammalian L1) |
| Description | Mouse Monoclonal |
| Gene | L1CAM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab24345 (1/500) immunostaining L1CAM in 4% PFA-fixed rat cerebellum tissue. ML = molecular layer and FT = fiber tract of the cerebellum.
![Anti-L1CAM antibody [2C2] (ab24345) at 1/1000 dilution + 30ug CNS proteinPerformed under reducing conditions.Observed band size : 200 kDa (why is the actual band size different from the predicted?)Additional bands at : 60-80 kDa (possible cleavage fragment).Martin Grumet, Rutgers University, United States](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14454_ab24345_2.jpg)
Anti-L1CAM antibody [2C2] (ab24345) at 1/1000 dilution + 30ug CNS proteinPerformed under reducing conditions.Observed band size : 200 kDa (why is the actual band size different from the predicted?)Additional bands at : 60-80 kDa (possible cleavage fragment).Martin Grumet, Rutgers University, United States
![Overlay histogram showing PC12 cells stained with ab24345 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24345, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14455_L1CAM-Primary-antibodies-ab24345-1.jpg)
Overlay histogram showing PC12 cells stained with ab24345 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24345, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Immunofluorescence analysis of COS7 cells transfected with full-length L1CAM (left) or truncated L1CAM (right), staining L1CAM (green) with ab24345. Cells were incubated with primary antibody (1/1000 in 1% goat serum + 0.3% Triton X-100 in PBS) and incubated overnight at 4°C. An AlexaFluor®488-conjugated anti-mouse IgG (1/700) was used as the secondary antibody. Nuclei were counterstained with bisbenzimide (blue).
Product References
Cancer stemness in Apc- vs. Apc/KRAS-driven intestinal tumorigenesis. - Cancer stemness in Apc- vs. Apc/KRAS-driven intestinal tumorigenesis.
Ghazvini M, Sonneveld P, Kremer A, Franken P, Sacchetti A, Atlasi Y, Roth S, Joosten R, Smits R, Fodde R. PLoS One. 2013 Sep 17;8(9):e73872.
L1CAM binds ErbB receptors through Ig-like domains coupling cell adhesion and - L1CAM binds ErbB receptors through Ig-like domains coupling cell adhesion and
Donier E, Gomez-Sanchez JA, Grijota-Martinez C, Lakoma J, Baars S, Garcia-Alonso L, Cabedo H. PLoS One. 2012;7(7):e40674.
Conditional deletion of NRSF in forebrain neurons accelerates epileptogenesis in - Conditional deletion of NRSF in forebrain neurons accelerates epileptogenesis in
Hu XL, Cheng X, Cai L, Tan GH, Xu L, Feng XY, Lu TJ, Xiong H, Fei J, Xiong ZQ. Cereb Cortex. 2011 Sep;21(9):2158-65.
Perisomatic GABAergic innervation in prefrontal cortex is regulated by ankyrin - Perisomatic GABAergic innervation in prefrontal cortex is regulated by ankyrin
Guan H, Maness PF. Cereb Cortex. 2010 Nov;20(11):2684-93.
Therapeutic antibodies to human L1CAM: functional characterization and - Therapeutic antibodies to human L1CAM: functional characterization and
Wolterink S, Moldenhauer G, Fogel M, Kiefel H, Pfeifer M, Luttgau S, Gouveia R, Costa J, Endell J, Moebius U, Altevogt P. Cancer Res. 2010 Mar 15;70(6):2504-15.
The differential role of L1 in ovarian carcinoma and normal ovarian surface - The differential role of L1 in ovarian carcinoma and normal ovarian surface
Zecchini S, Bianchi M, Colombo N, Fasani R, Goisis G, Casadio C, Viale G, Liu J, Herlyn M, Godwin AK, Nuciforo PG, Cavallaro U. Cancer Res. 2008 Feb 15;68(4):1110-8.
L1 interaction with ankyrin regulates mediolateral topography in the - L1 interaction with ankyrin regulates mediolateral topography in the
Buhusi M, Schlatter MC, Demyanenko GP, Thresher R, Maness PF. J Neurosci. 2008 Jan 2;28(1):177-88.
Activation of NMDA receptors promotes dendritic spine development through - Activation of NMDA receptors promotes dendritic spine development through
Tian L, Stefanidakis M, Ning L, Van Lint P, Nyman-Huttunen H, Libert C, Itohara S, Mishina M, Rauvala H, Gahmberg CG. J Cell Biol. 2007 Aug 13;178(4):687-700. Epub 2007 Aug 6.
L1 mediated homophilic binding and neurite outgrowth are modulated by alternative - L1 mediated homophilic binding and neurite outgrowth are modulated by alternative
Jacob J, Haspel J, Kane-Goldsmith N, Grumet M. J Neurobiol. 2002 Jun 5;51(3):177-89.
Nr-CAM expression in the developing mouse nervous system: ventral midline - Nr-CAM expression in the developing mouse nervous system: ventral midline
Lustig M, Erskine L, Mason CA, Grumet M, Sakurai T. J Comp Neurol. 2001 May 21;434(1):13-28.