All lanes : Anti-L3MBTL3 antibody (ab68117) at 1/500 dilutionLane 1 : L3MBTL3 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Immunofluorescence analysis of HeLa cells using purified ab68117 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
ab68117 used in Immunoprecipitation.Whole cell lysate from cells overexpressing L3MBTL3 tagged with GFP was loaded at 250000µg.Protein G was used in the Immunoprecipitation step.ab68117 used at 20µl/mg lysate.The western blot antibody was a rat anti-GFP polyclonal conjugated to agarose, used at a 1/5000 dilution.See Abreview