Anti-Lactate Dehydrogenase antibody
| Name | Anti-Lactate Dehydrogenase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2101 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB ELISA Conjugation ICC/IF ICC/IF IHC-F |
| Species Reactivities | Rat, Rabbit, Guinea Pig, Human, Potato |
| Antigen | Lactate Dehydrogenase [Rabbit Muscle] |
| Description | Goat Polyclonal |
| Gene | LDHA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2101 staining Lactate Dehydrogenase in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Rats were anesthetized and intracardially perfused with 500 ml of normal saline at room temperature, followed by 500 ml of ice-cold, freshly made 4% paraformaldehyde in phosphate buffer (PB, 0.1 M, pH 7.4). Alexa Fluor® 546 conjugated anti goat antibody was used as secondary.
ICC/IF image of ab2101 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2101, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent - Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent
Iguchi M, Kujuro Y, Okatsu K, Koyano F, Kosako H, Kimura M, Suzuki N, Uchiyama S, Tanaka K, Matsuda N. J Biol Chem. 2013 Jul 26;288(30):22019-32.
Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial - Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial
Hoshino A, Mita Y, Okawa Y, Ariyoshi M, Iwai-Kanai E, Ueyama T, Ikeda K, Ogata T, Matoba S. Nat Commun. 2013;4:2308.
Mitochondrial and plasma membrane lactate transporter and lactate dehydrogenase - Mitochondrial and plasma membrane lactate transporter and lactate dehydrogenase
Hussien R, Brooks GA. Physiol Genomics. 2011 Mar 16;43(5):255-64. doi:
A novel type of E3 ligase for the Ufm1 conjugation system. - A novel type of E3 ligase for the Ufm1 conjugation system.
Tatsumi K, Sou YS, Tada N, Nakamura E, Iemura S, Natsume T, Kang SH, Chung CH, Kasahara M, Kominami E, Yamamoto M, Tanaka K, Komatsu M. J Biol Chem. 2010 Feb 19;285(8):5417-27.
PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged - PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged
Matsuda N, Sato S, Shiba K, Okatsu K, Saisho K, Gautier CA, Sou YS, Saiki S, Kawajiri S, Sato F, Kimura M, Komatsu M, Hattori N, Tanaka K. J Cell Biol. 2010 Apr 19;189(2):211-21.
Physiological role of the cellular prion protein (PrPc): protein profiling study - Physiological role of the cellular prion protein (PrPc): protein profiling study
Ramljak S, Asif AR, Armstrong VW, Wrede A, Groschup MH, Buschmann A, Schulz-Schaeffer W, Bodemer W, Zerr I. J Proteome Res. 2008 Jul;7(7):2681-95.
Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in - Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in
Lemire J, Mailloux RJ, Appanna VD. PLoS One. 2008 Feb 6;3(2):e1550.
Evidence for the mitochondrial lactate oxidation complex in rat neurons: - Evidence for the mitochondrial lactate oxidation complex in rat neurons:
Hashimoto T, Hussien R, Cho HS, Kaufer D, Brooks GA. PLoS One. 2008 Aug 13;3(8):e2915.
Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular - Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular
Kim JW, Gao P, Liu YC, Semenza GL, Dang CV. Mol Cell Biol. 2007 Nov;27(21):7381-93. Epub 2007 Sep 4.
Homeostatic levels of p62 control cytoplasmic inclusion body formation in - Homeostatic levels of p62 control cytoplasmic inclusion body formation in
Komatsu M, Waguri S, Koike M, Sou YS, Ueno T, Hara T, Mizushima N, Iwata J, Ezaki J, Murata S, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T, Uwayama J, Warabi E, Yoshida H, Ishii T, Kobayashi A, Yamamoto M, Yue Z, Uchiyama Y, Kominami E, Tanaka K. Cell. 2007 Dec 14;131(6):1149-63.