Anti-Lactate Dehydrogenase antibody
| Name | Anti-Lactate Dehydrogenase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55433 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-335 of Human Lactate Dehydrogenase |
| Description | Mouse Monoclonal |
| Gene | LDHA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab55433 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55433, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14524_ab55433-4-ab55433FC.jpg)
Overlay histogram showing HeLa cells stained with ab55433 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55433, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Lactate Dehydrogenase antibody (ab55433) used in immunofluorescence at 10ug/ml on HeLa cells.
Lactate Dehydrogenase antibody (ab55433) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.
