![All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/mlLane 1 : LadderLane 2 : Human liver homogenate at 15 µgLane 3 : Human heart homogenate at 15 µgLane 4 : Rat liver homogenate at 15 µgLane 5 : Rat heart homogenate at 15 µgLane 6 : Mouse liver homogenate at 15 µgLane 7 : Mouse heart homogenate at 15 µgLane 8 : LadderLane 9 : Human fibroblast whole cell lysate at 15 µgLane 10 : HeLa whole Cell Lysate at 15 µgLane 11 : HepG2 whole cell lysate at 15 µgLane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µgLane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µgLane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µgLane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µgSecondaryGoat polyclonal to Mouse IgG - AP at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14530_Lactate-Dehydrogenase-Primary-antibodies-ab135396-6.jpg)
All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/mlLane 1 : LadderLane 2 : Human liver homogenate at 15 µgLane 3 : Human heart homogenate at 15 µgLane 4 : Rat liver homogenate at 15 µgLane 5 : Rat heart homogenate at 15 µgLane 6 : Mouse liver homogenate at 15 µgLane 7 : Mouse heart homogenate at 15 µgLane 8 : LadderLane 9 : Human fibroblast whole cell lysate at 15 µgLane 10 : HeLa whole Cell Lysate at 15 µgLane 11 : HepG2 whole cell lysate at 15 µgLane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µgLane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µgLane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µgLane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µgSecondaryGoat polyclonal to Mouse IgG - AP at 1/3000 dilution
Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.
Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml. Detection utilised Alexa 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution.
Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 µg/ml. The gel was stained with Coomassie brilliant blue G.
In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS.