![All lanes : Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLane 5 : SK-N-MC cell lysateLane 6 : RD cell lysateLane 7 : IMR32 cell lysateObserved band size : 30 kDa (why is the actual band size different from the predicted?)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14535_Lactate-Dehydrogenase-Primary-antibodies-ab85326-1.jpg)
All lanes : Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLane 5 : SK-N-MC cell lysateLane 6 : RD cell lysateLane 7 : IMR32 cell lysateObserved band size : 30 kDa (why is the actual band size different from the predicted?)
![Overlay histogram showing HeLa cells stained with ab85326 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85326, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14536_Lactate-Dehydrogenase-Primary-antibodies-ab85326-5.jpg)
Overlay histogram showing HeLa cells stained with ab85326 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85326, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.