ab112996 at 2ug/ml staining Lactate Dehydrogenase B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
ab112996 at 1ug/ml staining Lactate Dehydrogenase B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
Isolation of LDHB using ab112996 by immunoprecipitation. The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.