Anti-Lactate Dehydrogenase B antibody [EP1565Y]

• Supplier: Abcam
• Catalog: ab53292
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1565Y
• Applications: IHC-F WB IP ICC/IF FC IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Lactate Dehydrogenase B aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: LDHB
• Conjugate: Unconjugated

Product images

Anti-Lactate Dehydrogenase B antibody [EP1565Y] (ab53292) + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP

Human heart muscle labeled with ab53292 at 1/100 - 1/250 dilutionHuman heart muscle labelled with ab53292 at 1/100 - 1/250 dilution

ICC/IF image of ab53292 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53292, neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Overlay histogram showing HeLa cells stained with ab53292 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53292, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

beta-alanine suppresses malignant breast epithelial cell aggressiveness through - beta-alanine suppresses malignant breast epithelial cell aggressiveness through

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Lactate dehydrogenase B is required for the growth of KRAS-dependent lung - Lactate dehydrogenase B is required for the growth of KRAS-dependent lung

McCleland ML, Adler AS, Deming L, Cosino E, Lee L, Blackwood EM, Solon M, Tao J, Li L, Shames D, Jackson E, Forrest WF, Firestein R. Clin Cancer Res. 2013 Feb 15;19(4):773-84.

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An integrated genomic screen identifies LDHB as an essential gene for - An integrated genomic screen identifies LDHB as an essential gene for

McCleland ML, Adler AS, Shang Y, Hunsaker T, Truong T, Peterson D, Torres E, Li L, Haley B, Stephan JP, Belvin M, Hatzivassiliou G, Blackwood EM, Corson L, Evangelista M, Zha J, Firestein R. Cancer Res. 2012 Nov 15;72(22):5812-23.

The proteomics of colorectal cancer: identification of a protein signature - The proteomics of colorectal cancer: identification of a protein signature

O'Dwyer D, Ralton LD, O'Shea A, Murray GI. PLoS One. 2011;6(11):e27718.

Loss of lactate dehydrogenase B subunit expression is correlated with tumour - Loss of lactate dehydrogenase B subunit expression is correlated with tumour

Liao AC, Li CF, Shen KH, Chien LH, Huang HY, Wu TF. Pathology. 2011 Dec;43(7):707-12.