Anti-Lactate Dehydrogenase Isoenzyme V antibody [EPR1564]

• Supplier: Abcam
• Catalog: ab101562
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1564
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human Lactate Dehydrogenase Isoenzyme V
• Description: Rabbit Monoclonal
• Gene: LDHA
• Conjugate: Unconjugated

Product images

All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody [EPR1564] (ab101562) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : A375 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.

Staining of Human Lactate Dehydrogenase Isoenzyme V in a Formalin/PFA-fixed paraffin-embedded section of Human Muscle using ab101526 at a dilution of 1/500.

Overlay histogram showing HeLa cells stained with ab101562 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab101562, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunofluorescence analysis of rat primary oligodendrocytes, staining Lactate Dehydrogenase Isoenzyme V with ab101562. Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PNS) for 14 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/2000) was used as the secondary antibody.See Abreview

Product References

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Hyperpolarized 13C spectroscopic imaging informs on hypoxia-inducible factor-1 - Hyperpolarized 13C spectroscopic imaging informs on hypoxia-inducible factor-1

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