Western blot analysis of c-Jun p39 expression in untreated (A,C) and phorbol ester-induced (B,D) NIH/3T3 nuclear extracts. Antibodies tested include c-Jun (D): sc-44 (A,B) and c-Jun (N): sc-45 (C,D).
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected (A) and human c-Jun transfected (B) CHO whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected: sc-110760 (A) and human c-Jun transfected: sc-110759 (B) 293 whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125069 (B) 293T whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected: sc-117752 (A) and mouse c-Jun transfected: sc-125070 (B) 293T whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected 293T: sc-117752 (A), mouse c-Jun transfected 293T: sc-125071 (B) and NIH/3T3 (C) whole cell lysates.
c-Jun (N): sc-45. Western blot analysis of c-Jun expression in non-transfected CHO: sc-117750 (A), human c-Jun transfected CHO: sc-110019 (B) and NIH/3T3 (C) whole cell lysates.
Western blot analysis of human recombinant c-Jun (A,C) and human recombinant c-Jun phosphorylated by human recombinant JNK1 (B,D) fusion proteins. Antibodies tested include c-Jun (N): sc-45 (A,B) and p-c-Jun (KM-1): sc-822 (C,D).
Anisomycin: sc-3524. Intracellular FCM analysis of Anisomycin (sc-3524) induced c-Jun serine-63 phosphorylation: Pink dotted lines represent rabbit IgG PE stained Anisomycin (sc-3524) treated NIH/3T3 cells. Green line histograms represent untreated NIH/3T3 cells. Solid orange histograms represent Anisomycin (sc-3524) treated NIH/3T3 cells. Antibody tested: c-Jun (N) PE: sc-45 PE.
c-Jun (N)-G: sc-45-G. Western blot analysis of c-Jun expression in CCRF-CEM (A) and C6 (B) whole cell lysates.