Anti-Lamin B1 antibody [ZL-5]
| Name | Anti-Lamin B1 antibody [ZL-5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab20396 |
| Prices | $394.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | ZL-5 |
| Applications | FC ICC/IF ICC/IF WB IP ELISA |
| Species Reactivities | Mouse, Rat, Chicken, Human |
| Antigen | Recombinant human Lamin B |
| Description | Mouse Monoclonal |
| Gene | LMNB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Lamin B1 antibody [ZL-5] (ab20396) at 0.2 µg/ml + HeLa cell lysates](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14852_ab20396-221306-ab20396.jpg)
Anti-Lamin B1 antibody [ZL-5] (ab20396) at 0.2 µg/ml + HeLa cell lysates
![Overlay histogram showing HeLa cells stained with ab20396 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20396, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave no signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14853_Lamin-B1-Primary-antibodies-ab20396-1.jpg)
Overlay histogram showing HeLa cells stained with ab20396 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20396, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave no signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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