Anti-Lamin B1 antibody - Nuclear Envelope Marker
| Name | Anti-Lamin B1 antibody - Nuclear Envelope Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab16048 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F WB IHC-P |
| Species Reactivities | Mouse, Rat, Human, Pig, Xenopus, Deer, Chicken, Zebrafish |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse Lamin B1 |
| Description | Rabbit Polyclonal |
| Gene | LMNB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Performed under reducing conditions.
Performed under reducing conditions.
ICC/IF image of ab16048 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16048, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Performed under reducing conditions.
Performed under reducing conditions.
Human and mouse cells stained with ab16048 (1/500). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS.A: HeLa cells + ab16048 (green)B: HeLa cells counterstained with DAPI (blue)C: 3T3 cells + ab16048 (green)D: 3T3 cells counterstained with DAPI (blue)
Performed under reducing conditions.
Human and mouse cells stained with ab16048 (1/500). The cells were fixed and permeabilized in 4% formaldehyde, 0.2% Tritonm X100 for 10 minutes at room temperature, then washed 3x in PBS.A: HeLa cells + ab16048 (green)B: HeLa cells counterstained with DAPI (blue)C: 3T3 cells + ab16048 (green)D: 3T3 cells counterstained with DAPI (blue)
ab16048 staining Lamin B1 in Pig PAE cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 3% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilution + Pancreatic cell line - whole cell lysate at 20 µgSecondaryHRP conjugated goat anti-rabbit antibody at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab16048 staining Lamin B1 in human infantile fibromatosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 3 hours at room temperature; antigen retrieval was by heat mediation in Tris pH9. Samples were incubated with primary antibody (1/100 in TBS + 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
IHC image of Lamin B1 staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16048, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilutionLane 1 : Pig PAE whole cell lysateLane 2 : Pig PAE whole cell lysateLysates/proteins at 50 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
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Dynamic localization of glucokinase and its regulatory protein in hypothalamic - Dynamic localization of glucokinase and its regulatory protein in hypothalamic
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