Anti-Laminin 1+2 antibody

• Supplier: Abcam
• Catalog: ab7463
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F B/N IHC-P WB ICC/IF ICC/IF IHC-F
• Species Reactivities: Mouse, Rat, Sheep, Rabbit, Human
• Antigen: Full length native protein (purified) (Mouse EHS tumour)
• Description: Rabbit Polyclonal
• Gene: LAMA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Laminin 1+2 antibody (ab7463) at 1 µg/mlLane 1 : EHSLane 2 : HeartObserved band size : 200,400 kDa (why is the actual band size different from the predicted?)

Ab7463 staining Laminin in rat spinal cord and dorsal root by immunohistochemistry using paraformaldehyde/paraffin embedded tissue. Pepsin antigen retrieval was performed on this tissue sample.

ab7463 staining Laminin 1+2 in human MDA-MB-231 by ICC/IF (Immunocytochemistry/ Immunofluorescence). The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 buffer and blocked in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary.

Product References

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