Anti-LAP2 alpha antibody
| Name | Anti-LAP2 alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5162 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant His tagged fragment, corresponding to amino acids 188 - 693 of Human LAP 2 alpha |
| Description | Rabbit Polyclonal |
| Gene | TMPO |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab5162 at a dilution of 1/250 staining LAP 2 alpha in HeLa cells by immunofluorescence.A = ab5162, 1/250B = DNAC = A and B merged
ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL).
![LAP2 alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to LAP2 alpha (ab5162) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5162.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 83kDa: LAP2 alpha.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/15098_LAP2-alpha-Primary-antibodies-ab5162-3.jpg)
LAP2 alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to LAP2 alpha (ab5162) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5162.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 83kDa: LAP2 alpha.
Product References
Lamin B1 fluctuations have differential effects on cellular proliferation and - Lamin B1 fluctuations have differential effects on cellular proliferation and
Dreesen O, Chojnowski A, Ong PF, Zhao TY, Common JE, Lunny D, Lane EB, Lee SJ, Vardy LA, Stewart CL, Colman A. J Cell Biol. 2013 Mar 4;200(5):605-17.
Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric - Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric
Araki K, Kawauchi K, Hirata H, Yamamoto M, Taya Y. Elife. 2013 Dec 3;2:e01228.
Molecular characterization of the host defense activity of the barrier to - Molecular characterization of the host defense activity of the barrier to
Ibrahim N, Wicklund A, Wiebe MS. J Virol. 2011 Nov;85(22):11588-600.
Wild-type levels of human immunodeficiency virus type 1 infectivity in the - Wild-type levels of human immunodeficiency virus type 1 infectivity in the
Shun MC, Daigle JE, Vandegraaff N, Engelman A. J Virol. 2007 Jan;81(1):166-72. Epub 2006 Oct 11.