Anti-LAP2 alpha antibody [3A3]

Name	Anti-LAP2 alpha antibody [3A3]
Supplier	Abcam
Catalog	ab66588
Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	3A3
Applications	ICC/IF ICC/IF WB
Species Reactivities	Human
Antigen	Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human LAP2 alpha
Description	Mouse Monoclonal
Gene	TMPO
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-LAP2 alpha antibody [3A3] (ab66588) at 10 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (post mitotic stage) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

ICC/IF image of ab66588 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66588, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).