All lanes : Anti-LARP7 antibody (ab134757) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cells at 50 µgLane 2 : Whole cell lysate from HeLa cells at 15 µgLane 3 : Whole cell lysate from 293T cells at 50 µgLane 4 : Whole cell lysate from Jurkat cells at 50 µgLane 5 : Whole cell lysate from mouse NIH3T3 cells at 50 µgdeveloped using the ECL technique
Lane 1: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of LARP7. Lane 2: Detection of Human LARP7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134757 at 6 µg/mg lysate for IP. Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG. Subsequent Western blot detection used ab134757 at 1 µg/ml. Gel concentration 4-20% Developed using the ECL technique with an exposure time of 10 seconds.