All lanes : Anti-LECT2 antibody [EPR14464(2)] (ab196015) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Human fetal liver lysateLysates/proteins at 20 µg per lane.Secondaryanti-rabbit IgG (HRP), specific to the non-reduced form of IgG HRP at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-LECT2 antibody [EPR14464(2)] (ab196015) at 1/1000 dilutionLane 1 : Human fetal lung lysateLane 2 : Human adult testis lysateLysates/proteins at 20 µg per lane.Secondaryanti-rabbit IgG (HRP), specific to the non-reduced form of IgG HRP at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-LECT2 antibody [EPR14464(2)] (ab196015) at 1/10000 dilutionLane 1 : mouse liver lysateLane 2 : rat liver lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling LECT2 with ab196015 at a 1/8000 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (ab97051) was used at a 1/500 dilution. In the negative control PBS was used instead of primary antibody.
Immunohistochemical analysis of paraffin-embedded, rat cerebral cortex tissue labeling LECT2 with ab196015 at a 1/8000 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (ab97051) was used at a 1/500 dilution. In the negative control PBS was used instead of primary antibody.
Immunofluorescence analysis of paraformaldehyde-fixed HepG2 cells labeling LECT2 with ab196015 at a 1/900 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/500 dilution. In blue DAPI staining.