Lane 1 : Anti-Leptin Receptor antibody [EPR911(N)] - C-terminal (ab177469) at 1/2000 dilution (purified)Lane 2 : Anti-Leptin Receptor antibody [EPR911(N)] - C-terminal (ab177469) at 1/2000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-Leptin Receptor antibody [EPR911(N)] - C-terminal (ab177469) at 2000 µg (purified)Lane 1 : Human fetal brain tissue lysateLane 2 : U87-MG cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution
All lanes : Anti-Leptin Receptor antibody [EPR911(N)] - C-terminal (ab177469) at 1/1000 dilution (unpurified)Lane 1 : Fetal liver lysateLane 2 : Fetal brain lysateLane 3 : U87MG lysateLane 4 : SH-SY5Y lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique