Anti-Leukotriene A4 hydrolase antibody [EPR5713]

• Supplier: Abcam
• Catalog: ab133512
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5713
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Description: Rabbit Monoclonal
• Gene: LTA4H
• Conjugate: Unconjugated

Product images

Overlay histogram showing Jurkat cells stained with ab133512 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133512, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Leukotriene A4 hydrolase antibody [EPR5713] (ab133512) at 1/10000 dilutionLane 1 : HeLa cell lysate Lane 2 : 293T cell lysateLane 3 : T47D cell lysateLane 4 : A549 cell lysate Lysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilution

All lanes : Anti-Leukotriene A4 hydrolase antibody [EPR5713] (ab133512) at 1/5000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Mouse heart tissue lysateLane 3 : Mouse kidney tissue lysateLane 4 : Mouse spleen tissue lysateLane 5 : Rat brain tissue lysateLane 6 : Rat heart tissue lysateLane 7 : Rat kidney tissue lysateLane 8 : Rat spleen tissue lysate

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Leukotriene A4 hydrolase with ab133512 at 1/250 dilution.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

Immunohistochemical localization of key arachidonic acid metabolism enzymes - Immunohistochemical localization of key arachidonic acid metabolism enzymes

Lin HN, O'Connor JP. PLoS One. 2014 Feb 7;9(2):e88423.