All lanes : Anti-Leukotriene B4 Receptor antibody (ab82851) at 1 µg/mlLane 1 : Thyroid (Human) Whole Cell Lysate - adult normal tissue (ab29636)Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 3 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lysates/proteins at 20 µg per lane.SecondaryLanes 1 - 2 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab82851 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82851, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.