Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab96178 antibody at 1:500 dilution.
Anti-LIMCH1 antibody (ab96178) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg
ICC/IF image of ab96178 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96178, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-LIMCH1 antibody (ab96178) at 1/1000 dilution + H1299 whole cell lysate at 30 µg