Anti-LIMK1 antibody
| Name | Anti-LIMK1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab81046 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Rat, Human, Mouse, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human LIMK1 |
| Description | Rabbit Polyclonal |
| Gene | LIMK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-LIMK1 antibody (ab81046) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Rat Cortex Tissue Lysate Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 4 : SK N BE (Human neuroblastoma) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab81046 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab81046 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81046, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of - LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of
Kim JE, Ryu HJ, Kim MJ, Kang TC. Cell Death Differ. 2014 Jul;21(7):1036-49.
Estradiol dose-dependent regulation of membrane estrogen receptor-alpha, - Estradiol dose-dependent regulation of membrane estrogen receptor-alpha,
Mahavongtrakul M, Kanjiya MP, Maciel M, Kanjiya S, Sinchak K. Endocrinology. 2013 Sep;154(9):3251-60.