Flow cytometric analysis of Human embryonic stem cells labeling Lin28 with ab184681 at 1/50 dilution (green). The isotype control at 1/50 dilution is shown in black.
Anti-Lin28 antibody [GT717] (ab184681) at 1/1000 dilution + Human ESC whole cell lysate/extract at 30 µg
Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling Lin28 with ab184681 at 1/100 dilution.
Anti-Lin28 antibody [GT717] (ab184681) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
All lanes : Anti-Lin28 antibody [GT717] (ab184681) at 1/1000 dilutionLane 1 : Non-transfected HeLa lysatesLane 2 : Lin28-transfected HeLa lysatesLysates/proteins at 30 µg per lane.
Immunofluorescence analysis of 4% paraformaldehyde-fixed Human embryonic stem cell cells labeling Lin28 with ab184681 at 1/200 dilution (green). The slide on the right is counterstained with Hoechst 33342 (blue).