Anti-Lin28B antibody

• Supplier: Abcam
• Catalog: ab71415
• Prices: $384.00
• Sizes: 400 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P FC WB
• Species Reactivities: Human, Mouse
• Antigen: Synthetic peptide between amino acids of 105-135 of human Lin28B, conjugated to KLH
• Description: Rabbit Polyclonal
• Gene: LIN28B
• Conjugate: Unconjugated

Product images

Anti-Lin28B antibody (ab71415) at 1/1000 dilution + K562 cell line lysates at 35 µgSecondaryHRP goat anti-rabbit (H+L) at 1/5000 dilution

Immunohistochemical staining of Lin28B in Human testis tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71415 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human spleen tissue labelling Lin28B with ab71415 at 1/50. A peroxidase conjugated secondary antibody was used followed by AEC staining.

Immunocytochemisty/Immunofluorescence analysis of HepG2 cells labelling Lin28B (green) with ab71415. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstained with DAPI (blue)

Flow cytometry analysis of HL-60 cells labelling Lin28B (green) with ab71415 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product References

miR-26a enhances miRNA biogenesis by targeting Lin28B and Zcchc11 to suppress - miR-26a enhances miRNA biogenesis by targeting Lin28B and Zcchc11 to suppress

Fu X, Meng Z, Liang W, Tian Y, Wang X, Han W, Lou G, Wang X, Lou F, Yen Y, Yu H, Jove R, Huang W. Oncogene. 2014 Aug 21;33(34):4296-306.

Lin28b promotes head and neck cancer progression via modulation of the - Lin28b promotes head and neck cancer progression via modulation of the

Alajez NM, Shi W, Wong D, Lenarduzzi M, Waldron J, Weinreb I, Liu FF. Oncotarget. 2012 Dec;3(12):1641-52.

Human ovarian cancer stem/progenitor cells are stimulated by doxorubicin but - Human ovarian cancer stem/progenitor cells are stimulated by doxorubicin but

Meirelles K, Benedict LA, Dombkowski D, Pepin D, Preffer FI, Teixeira J, Tanwar PS, Young RH, MacLaughlin DT, Donahoe PK, Wei X. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2358-63. doi:

MicroRNA let-7c suppresses androgen receptor expression and activity via - MicroRNA let-7c suppresses androgen receptor expression and activity via

Nadiminty N, Tummala R, Lou W, Zhu Y, Zhang J, Chen X, eVere White RW, Kung HJ, Evans CP, Gao AC. J Biol Chem. 2012 Jan 6;287(2):1527-37.

High expression of Lin28 is associated with tumour aggressiveness and poor - High expression of Lin28 is associated with tumour aggressiveness and poor

Hamano R, Miyata H, Yamasaki M, Sugimura K, Tanaka K, Kurokawa Y, Nakajima K, Takiguchi S, Fujiwara Y, Mori M, Doki Y. Br J Cancer. 2012 Apr 10;106(8):1415-23.

MiR-181 mediates cell differentiation by interrupting the Lin28 and let-7 - MiR-181 mediates cell differentiation by interrupting the Lin28 and let-7

Li X, Zhang J, Gao L, McClellan S, Finan MA, Butler TW, Owen LB, Piazza GA, Xi Y. Cell Death Differ. 2012 Mar;19(3):378-86.

An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links - An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links

Iliopoulos D, Hirsch HA, Struhl K. Cell. 2009 Nov 13;139(4):693-706.