Immunohistochemistry Image of Human Spleen (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry Image of Human Testis (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry Image of Human Testis (formalin-fixed, paraffin-embedded) stained with Lin28B antibody (ab115698) at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Anti-Lin28B antibody (ab115698) at 1/1000 dilution + HL60 cell line lysate at 35 µgSecondaryHRP goat anti-rabbit (H+L) at 1/5000 dilution
Overlay histogram showing HL-60 cells stained with ab115698 (green line). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab115698, 1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody used was FITC conjugated goat anti-rabbit IgG at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions. Acquisition of >10,000 events was performed.