Anti-LIN9 antibody
| Name | Anti-LIN9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab62329 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human, Chicken, Xenopus, Zebrafish, Monkey |
| Antigen | Synthetic peptide derived from within residues 500 to the C-terminus of Human LIN9 |
| Blocking Peptide | Human LIN9 peptide |
| Description | Rabbit Polyclonal |
| Gene | LIN9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-LIN9 antibody (ab62329) at 1 µg/mlLane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 3 : Testis (Mouse) Tissue Lysate Lane 4 : Testis (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab62329 stained HepG2 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62329, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Product References
The forkhead transcription factor FOXM1 controls cell cycle-dependent gene - The forkhead transcription factor FOXM1 controls cell cycle-dependent gene
Chen X, Muller GA, Quaas M, Fischer M, Han N, Stutchbury B, Sharrocks AD, Engeland K. Mol Cell Biol. 2013 Jan;33(2):227-36.
LIN9, a subunit of the DREAM complex, regulates mitotic gene expression and - LIN9, a subunit of the DREAM complex, regulates mitotic gene expression and
Esterlechner J, Reichert N, Iltzsche F, Krause M, Finkernagel F, Gaubatz S. PLoS One. 2013 May 7;8(5):e62882.
Polo-like kinase 4 transcription is activated via CRE and NRF1 elements, - Polo-like kinase 4 transcription is activated via CRE and NRF1 elements,
Fischer M, Quaas M, Wintsche A, Muller GA, Engeland K. Nucleic Acids Res. 2014 Jan;42(1):163-80.
p53 can repress transcription of cell cycle genes through a - p53 can repress transcription of cell cycle genes through a
Quaas M, Muller GA, Engeland K. Cell Cycle. 2012 Dec 15;11(24):4661-72.