All lanes : Anti-LINGO3 antibody [EPR9635] (ab169772) at 1/1000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : U87-MG cell lysateLane 3 : 293T cell lysateLane 4 : NCI-H460 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunofluorescent analysis of 293T cells labeling LINGO3 with ab169772 at 1/250 dilution (green). DAPI nuclear staining (blue).
Flow cytometry analysis of permeabilized SH-SY5Y cells labeling LINGO3 with ab169772 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab169772.